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inverted confocal microscope nikon eclipse ts2  (Nikon)


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    Nikon inverted confocal microscope nikon eclipse ts2
    Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown ( N =3, n =196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown ( N =3, n =12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron <t>microscope</t> show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.
    Inverted Confocal Microscope Nikon Eclipse Ts2, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inverted confocal microscope nikon eclipse ts2/product/Nikon
    Average 90 stars, based on 1 article reviews
    inverted confocal microscope nikon eclipse ts2 - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "Primary human neutrophils and monocytes migrate along endothelial cell boundaries to optimize search efficiency under static in vitro conditions"

    Article Title: Primary human neutrophils and monocytes migrate along endothelial cell boundaries to optimize search efficiency under static in vitro conditions

    Journal: Biology Open

    doi: 10.1242/bio.061704

    Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown ( N =3, n =196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown ( N =3, n =12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron microscope show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.
    Figure Legend Snippet: Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown ( N =3, n =196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown ( N =3, n =12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron microscope show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.

    Techniques Used: Cell Culture, Immunocytochemistry, Staining, Negative Control, Migration, Fluorescence, Transmigration Assay, Derivative Assay, Microscopy



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    inverted confocal microscope nikon eclipse ts2  (Nikon)
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    Nikon inverted confocal microscope nikon eclipse ts2
    Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown ( N =3, n =196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown ( N =3, n =12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron <t>microscope</t> show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.
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    eclipse ts2 inverted confocal microscope  (Nikon)
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    Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown ( N =3, n =196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown ( N =3, n =12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron <t>microscope</t> show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.
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    Image Search Results


    Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown ( N =3, n =196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown ( N =3, n =12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron microscope show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.

    Journal: Biology Open

    Article Title: Primary human neutrophils and monocytes migrate along endothelial cell boundaries to optimize search efficiency under static in vitro conditions

    doi: 10.1242/bio.061704

    Figure Lengend Snippet: Primary human monocytes cultured on top of monolayer of HUVECs migrate along cell–cell boundaries. (A) Primary human monocytes (Hoechst, red) enrich at cell–cell boundaries of HUVECs (α-Catenin, white). To the right, quantification of cells located at cell–cell boundaries (green) and on top of the cell (magenta) is shown ( N =3, n =196 cells). (B) Immuno-cytochemistry indicates that primary human monocytes transmigrate the HUVEC layer. Both the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (C) Statistical analysis of motion pattern for primary human monocytes and HUVECs. At the top, representative tracks are shown ( N =3, n =12 technical repeats). The dashed lines serve as a guidance to the eye. At the bottom, to the left, graph depicting the number of cells, normalized to the initial count, throughout the entire acquisition period. To the right, graph tracking speed over time. Each blue line represents a technical repeat. (D) Primary human monocytes (Hoechst, red) migrate along cell–cell boundaries (actin, white) of HUVECs. Only the nucleus of monocytes is labelled by Hoechst. (E) Motion tracks of human monocytes on top of HUVECs. At the top, Voronoi of endothelial layer (gray) as well as tracks of monocytes and of HUVECs are shown. Insets to the right are 70 µm×70 µm. Below, to the left, cross-correlation analysis shows enrichment of monocyte tracks along endothelial cell–cell boundaries identified by Vornoi tesselation. As negative control, to the bottom right, one channel was rotated by 180° prior to cross-correlation analysis. (F) Time-lapse of primary human monocytes (red) show different migration pattern. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (G) Primary human monocytes change fluorescence intensity of nucleus during transmigration. Both, the nuclei of monocytes (small) and HUVECs (large) are stained with Hoechst. (H) ER-HoxB8 derived monocytes/macrophages migrate along HUVEC boundaries. To the left, HUVECs (green) and tracks of ER-HoxB8 derived monocytes/macrophages (magenta) are shown. To the right, scanning electron microscope show ER-HoxB8 derived monocytes/macrophages at cell–cell boundaries of a confluent HUVEC sheet. Scale bars: (A,D,F,G) 20 µm, (B) 10 µm, (C,E) 100 µm, (H) 200 µm and 500 µm.

    Article Snippet: Cell migration was imaged using an inverted confocal microscope (Nikon, Eclipse Ts2) with a digital camera (Nikon DS-Fi2), using a 20× objective at 37°C and 5% CO 2 with a frame interval of 60 s. Using multi-frame imaging allowed the recording of multiple positions per channel (i.e. technical repeats).

    Techniques: Cell Culture, Immunocytochemistry, Staining, Negative Control, Migration, Fluorescence, Transmigration Assay, Derivative Assay, Microscopy